The Short Range Biological Standoff Detection System (SR-BSDS) is a multi-wavelength LIDAR system with both ultraviolet and infrared capability. A rugged azimuth and elevation scanning gimbal allows the system to autonomously scan for and monitor aerosol clouds while sensitive PMT and APD detectors convert return signals for real time data processing and display. This technology is being developed for the military by Fibertek. The SR-BSDS has successfully demonstrated the ability to autonomously detect and track a biological aerosol cloud while discriminating between biological and non-biological aerosols and hard targets. The UV wavelengths can provide near real time detection and discrimination of particulate clouds. The IR wavelength provides cloud detection, acquisition and tracking capability at longer ranges, which has been successfully demonstrated. An efficient frequency converted diode-pumped solid-state ultraviolet laser transmitter is utilized as the excitation source in the biological LIDAR system. Detection capabilities are based upon laser induced fluorescence (LIF) detected upon UV excitation of the constituents of biological aerosol clouds. The SR-BSDS utilizes dual receiver wavelength bands to distinguish biological aerosols of interest from background pollens and other aerosols. An additional eye safe infrared laser output improves initial detection of aerosol clouds. Thus, the multi-wavelength SR-BSDS has the ability to achieve early warning, tracking and discrimination of biological agent clouds combined with the UV capability to reduce false detections from battlefield interferents and improve sensitivity of detection in high aerosol background conditions. Field testing to date has demonstrated the system’s scanning, detection, discrimination and autonomous operation functions. During these tests numerous scenarios were evaluated. Cloud disseminations included a matrix of ranges, concentrations, dissemination techniques and simulants.